Character set from the PC88 eroge 妖獣教室 / Youjuu Kyoushitsu Gakuen spin off of the manga and OVA series. I know little to nothing about the game other than general plot line. I only identified the lead female character Kayo and her friend Miki who is only in the first part of this. The miko may be Kayo's cousin Miyuki who is also featured in episode 4 of the series.

All the characters from the screenshot were trained some had more material. The original game has a small resolution with pixels visible (very high quality art for the medium) most of the samples were modified to scale up and smooth the lines and remove mosaic. I haven't tried recreating the pixelized look. Given the single source material this does double as a style lora.

I like the style here, enjoyed the franchise, Toshio Maeda's stuff, and it had some solid monster transformations.

Kayo

Casual

Yoju_Kyousitu_Gakuen, 1girl, solo, kayo, bangs, brown eyes, red shirt, red skirt, white jacket, hair ribbons, short twin tails,

School Uniform

Yoju_Kyousitu_Gakuen, 1girl, solo, kayo, bangs, brown hair, short hair, brown eyes, hair ribbons, short twin tails, school uniform, blue blazer, white blouse, blue plaid skirt, red ribbon, looking at viewer

Miyuki

Miko

Yoju_Kyousitu_Gakuen, 1girl, solo, prstess, long hair, hime cut, dark blue eyes, dark blue hair, scarlet hakama, white kosode, long sleeves, silk vestment

School Uniform

Yoju_Kyousitu_Gakuen, 1girl, solo, prstess, long hair, hime cut, dark blue eyes, dark blue hair, seifuku, green collar, green skirt

Demon transformation

Yoju_Kyousitu_Gakuen, 1girl, solo, prstess, monster girl, long hair, white eyes, blank eyes, dark blue hair, nude, yelling, horns, pointed ears, brown skin, transformation, full transformation, warty skin, fangs, sharp teeth, bdy_tctle, open mouth, angry, nipples

Red haired girl

Yoju_Kyousitu_Gakuen, 1girl, solo, rprter, blue eyes, short hair, red hair, white tank top, pencil skirt, grey skirt, belt, blue jacket, necklace

Spider monster transformation (first stage)

Yoju_Kyousitu_Gakuen, 1girl, solo, rprter, monster girl, black eyes, pale green skin, short hair, red hair, nude, nipples, pointed ears, earrings, open mouth, sharp teeth, transforming, transformation, short extra limbs, torso spikes, hip spikes, floating hair

Spider monster transformation (second stage)

Yoju_Kyousitu_Gakuen, 1girl, solo, rprter, monster girl, black eyes, gray skin, short hair, red hair, nude, nipples, pointed ears, earrings, open mouth, sharp teeth, mandibles, circular mouth, full transformation, transformation, extra limbs, torso limbs, hip limbs, segmented body, navel, floating hair

Brunette girl

Yoju_Kyousitu_Gakuen, 1girl solo, bsnwm, tan blazer, white blouse, long tan skirt, glasses, brunette hair, hair up, bun

[A] Guy

Yoju_Kyousitu_Gakuen, 1boy, solo, black eyes, short hair, brown hair, white shirt, brown vest, jeans, watch

Miki

Yoju_Kyousitu_Gakuen, 1girl, solo, miki, short hair, brown hair, brown eyes, sitting, school uniform, blue blazer, white blouse, red ribbon

Nurse

Nurse outfit

Yoju_Kyousitu_Gakuen, 1girl solo, schnurse, dark blue eyes, dark blue hair, short hair, white lab coat, yellow blouse, green skirt, pantyhose, red earrings, red lipstick, ring, red bracelet

Lingerie

Yoju_Kyousitu_Gakuen, 1girl solo, schnurse, dark blue eyes, dark blue hair, short hair, red earrings, red lipstick, red lingerie, red mesh bra, garters, red stocking, red panties, red nail, ring, red bracelet, cord necklace

Demon Tentacle Monster

Yoju_Kyousitu_Gakuen, 1boy, monster, tentacle monster, blue-skinned male, tentacles, horns, fangs, blank eyes, white eyes, open mouth,