Unity debuted as a recurring character in the animated series Rick and Morty in Season 2, Episode 3, titled "Auto Erotic Assimilation," which aired on August 9, 2015. Voiced primarily by Christina Hendricks, with additional voices from various performers reflecting the entities it assimilates, Unity is depicted as a sentient hive mind capable of possessing and controlling the bodies of entire populations across planets. Genderless and pansexual, Unity manifests through diverse avatars of multiple races, genders, and species, allowing simultaneous interactions and control over assimilated societies to create utopian order and harmony.

Unity's personality combines benevolence, organizational prowess, and vulnerability to external influences, particularly in its complicated romantic history with Rick Sanchez. In its initial appearance, Unity reunites with Rick on a conquered planet, indulging in hedonistic activities that lead to temporary loss of control over the inhabitants, resulting in societal chaos. Recognizing Rick's destructive impact, Unity ultimately terminates the relationship through a series of farewell notes, highlighting themes of codependency and self-awareness, while leaving Rick in a state of profound despair.

Unity experiences further development in Season 7, Episode 3, "Air Force Wong," aired on November 5, 2023, where it reemerges with persistent attempts to reconnect with Rick, enlisting his aid alongside Dr. Wong to combat a rival hive mind influencing the U.S. President. This return portrays Unity as more assertive and concerned for Rick's well-being, yet underscores ongoing relational tensions and boundary issues. Additional explorations in tie-in comics, such as the 2019 one-shot Rick and Morty Presents: Unity, expand on its post-breakup pursuits, reinforcing its role as a complex entity grappling with individuality, control, and emotional attachment within the series' multiversal framework.